(1) There were many reports of bactericidal experiments on ozone related to bacterial reproductive bodies. When ozone was injected into the aseptic shield (0.7m3), it was observed that the killing rates of Escherichia coli were 97.5% and 100% after 20 minutes and 30 minutes of ozone action. The killing rates of Staphylococcus aureus were 93.7% and 100%. The killing rates of P. aeruginosa were 84.6% and 89.8%. Herbold et al. reported that when ozone gas was injected into flowing water at 20℃, E. coli could be killed 100% when the concentration of ozone in water reached 0.43mg/L. Bai Xiyao et al. also found that ozone aqueous solution has a strong bactericidal effect and a very fast speed, and the killing rate of E. coli and Staphylococcus aureus with a concentration of 0.3mg/L for 1 minute is 100%.

Under the condition of 34℃±1℃, 5.50mg/m3 ozone treatment for 45 minutes can kill all the black variant spores of Bacillus subtilis dripping in 100ml plastic bottle. Ouyang Chuan et al. continuously injected ozone gas into bacterial-infected culture water under dynamic experimental conditions, and found that it was not smelly. They found that when the ozone concentration in water was 8.87-4.61mg/L, it could kill 99.99% of the black variant spores of Bacillus subtilis in culture water for 3-10 minutes.
3 Pair of viruses Ozone can inactivate a variety of viruses and the speed is very fast 1 minute can inactivate 90% of the oocysts; Chlorine dioxide requires 1 hour of action, 80ppm of chlorine and 80ppm of chloramine need 90 minutes to achieve the same inactivation effect.
After the seawater containing 400,000-500,000 /ml of unicellular algae and protozoa were treated with ozone for 30 minutes, all the native plants and animals were found to be explosive dead by microscope observation. After the ozone-treated seawater was cultured for two weeks, no organisms were found and the water quality was clear and transparent. After 30 minutes of ozone treatment, the red red tide seawater was oxidized and turned white and transparent, and all noctiluca in the water were killed and pulverized.
Five ozone purification of water quality Ozone as a strong oxidizing agent has played a very important role in improving water quality. The harmful ammonia nitrogen, nitrite nitrogen, hydrogen sulfide and SO on in the aquaculture water are oxidized by ozone into non-toxic NO 3-, N 2, so 42 – and other organic and inorganic substances that cannot be biodegraded can also be oxidized into simple non-toxic substances to reduce BOD and COD.
6, ozone water generation 1 Air or oxygen after dehumidification, dust removal into a clean dry gas to the ozone generator, the ozone gas generated into the ozone water formation machine, and then the aquaculture water is also input to make the water fully atomized into non-fine small particles, increase the solubility of ozone in water and adhesion area; The production efficiency of ozone concentration of ozone water was improved. The solubility of ozone dissolved in water is greatly affected by water temperature and ozone concentration. The solubility of ozone dissolved in water increases when the temperature is low, and the water temperature at 0℃ is 3 times that at 30℃. The solubility of ozone increases linearly with the concentration of ozone gas. The ability of oxidation sterilization was greatly improved. For example, at the same time of sterilization, the ozone gas sterilization concentration ratio is 100, then the ozone water sterilization concentration ratio is reduced to 1, if the water activation is particularly good, the ozone water concentration ratio is reduced to about 0.2. 2 Ozone water treatment system structure A complete ozone treatment system should include water source, water source pre-treatment, ozone water treatment, purification post-treatment, production water and recycling and other parts.
The factors that affect the effect of ozone water disinfection and sterilization effect of ozone water are many, mainly: the concentration of ozone, the temperature of water, pH value, impurities and organisms in water, etc., the higher the concentration of odorization, the higher the concentration of residual ozone in water; Under the same ozone concentration, the lower the water temperature, the higher the solubility of ozone, the solubility of 22% at 30℃, 54% at 10℃ and 69% at 0℃, the pH value affects the oxidation capacity of ozone, the high pH value has a strong oxidation capacity, the influence of water turbidity below 5mg/l on ozone disinfection and sterilization is very small, when the turbidity of water is above 5mg/l, It has an effect on ozone sterilization. A considerable part of the amount of ozone is used for the oxidation and decomposition of organic matter and inorganic matter, so the water used to make ozone water should be clarified and filtered to remove impurities. When the impurities in the water increase by 1mg/l, it is necessary to use 2 ~ 4mg ozone for oxidation and decomposition, and the increase in ozone consumption is uneconomical.
The advantages and disadvantages of ozonization disinfection technology: 1, the effect of ozone disinfection is extremely strong, whether it is bacteria, viruses, or spores that have not been spore have a killing effect. 2, the killing speed is 600-3000 times that of chlorine, and its concentration is 0.000048 times that of chlorine when it has the same sterilization effect (killing E. coli rate is 99.9%). 3. The oxides produced in the ozone disinfection process are non-toxic, odorless and biodegradable substances. 4, ozone can be quickly decomposed into oxygen, does not produce secondary pollution, is called “clean disinfectant”. And improve the dissolved oxygen in aquaculture water. 5, ozone gas free state, disinfection will not produce dead corners. 6, ozone in the disinfection process through its oxidation flocculation on the water quality has a certain purification effect. 7, ozone disinfection technology can be used for aquaculture water drinking water seawater, sewage treatment can also be used for environmental objects and other disinfection applications. 8, ozone in the application, can only be produced in situ, so simple, complete and economic. Disadvantages: 1, no protective margin for the disinfected substance 2, ozone is a toxic gas excess will cause people’s respiratory system obstacles, which requires the use of sealing so that people do not stay in the environment of excessive ozone for too long.
Effects of residual ozone on aquatic organisms The toxic effects of ozone on fish are abnormal movement and respiration. Then it loses its balance, and then it swims and then it stops, when the fish’s side or belly is up. The period of inactivity gradually lengthens, and finally the fish die. Experiments have shown that fish can recover from abnormal movement and breathing symptoms, but if they lose their balance, they will inevitably die, even if they are moved into the right water. In the experiment, the residual ozone concentration as low as 0.9mg/L can kill fish, and the residual ozone concentration as low as 1.16mg/L can kill crayfish. At a residual ozone concentration of 1.25mg/L, microscopic plankton and benthic invertebrates were killed almost immediately.
First, the death of ozone is a natural death, ozone in 250nm ~ 320nm ultraviolet irradiation naturally decomposed into O2; The second is the combination of death, ozone is a strong oxidizer, unstable, can quickly decompose substances and substances react with the combination of death and speed is very fast.
In summary, ozone is an ideal sterilization disinfectant, sterilization spectrum is wide, rapid and thorough, which can save a lot of disinfectants and antimicrobials, and no secondary pollution, but also remove NH3-N, NO2-N and other waste, purification water decomposition of oxygen dissolved into the water to supplement the oxygen content in the water. In short, ozone treatment aquaculture water technology is an advanced water purification technology, which is incomparable to other chemical agents.