Ii-aquariums ezinkulu zikamasipala kunye ne-zoos zibulala iintsholongwane emanzini nge-ozone ukukhusela iintlobo ezahlukeneyo kusulelo kunye nezifo ezibangelwa ngamanzi.I-ozone itshabalalisa ibhaktheriya, yenza iintsholongwane zingasebenzi, kwaye i-oxidizes eziphilayo.Amanzi acocekileyo, acacileyo, kwaye akhuselekile. Akukho vumba, akukho ntsalela iyingozi, ulawulo olusebenzayo lwentsholongwane kunye nokucaca kwamanzi zizinto ezine eziphambili ezifunekayo.